Search results for "miRNA targets"

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Identification of Stress Associated microRNAs in Solanum lycopersicum by High-Throughput Sequencing

2019

Tomato (Solanum lycopersicum) is one of the most important crops around the world and also a model plant to study response to stress. High-throughput sequencing was used to analyse the microRNA (miRNA) profile of tomato plants undergoing five biotic and abiotic stress conditions (drought, heat, P. syringae infection, B. cinerea infection, and herbivore insect attack with Leptinotarsa decemlineata larvae) and one chemical treatment with a plant defence inducer, hexanoic acid. We identified 104 conserved miRNAs belonging to 37 families and we predicted 61 novel tomato miRNAs. Among those 165 miRNAs, 41 were stress-responsive. Reverse transcription quantitative PCR (RT-qPCR) was used to valida…

0106 biological sciences0301 basic medicineEstrèslcsh:QH426-470ATP-binding cassette transporter01 natural sciencesbehavioral disciplines and activitiesDNA sequencingdifferential expression03 medical and health sciencesDifferential expressionSolanum lycopersicummicroRNAGeneticsTomàquetsGeneGenetics (clinical)Abiotic componentGeneticsbiotic and abiotic stress responseHigh-throughput sequencingbiologyAbiotic stressfungi<i>Solanum lycopersicum</i>food and beverageshigh-throughput sequencingbiology.organism_classificationlcsh:Genetics030104 developmental biologyReal-time polymerase chain reactionmiRNAsBiotic and abiotic stress responseSolanumHexanoic acidhexanoic acidmiRNA targets010606 plant biology & botanyGenes
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Identification of Stress Associated microRNAs in

2019

Tomato (Solanum lycopersicum) is one of the most important crops around the world and also a model plant to study response to stress. High-throughput sequencing was used to analyse the microRNA (miRNA) profile of tomato plants undergoing five biotic and abiotic stress conditions (drought, heat, P. syringae infection, B. cinerea infection, and herbivore insect attack with Leptinotarsa decemlineata larvae) and one chemical treatment with a plant defence inducer, hexanoic acid. We identified 104 conserved miRNAs belonging to 37 families and we predicted 61 novel tomato miRNAs. Among those 165 miRNAs, 41 were stress-responsive. Reverse transcription quantitative PCR (RT-qPCR) was used to valida…

biotic and abiotic stress responsefungifood and beveragesHigh-Throughput Nucleotide Sequencinghigh-throughput sequencingbehavioral disciplines and activitiesArticledifferential expressionDroughtsMicroRNAsSolanum lycopersicumGene Expression Regulation PlantStress PhysiologicalmiRNAshexanoic acidmiRNA targetsPlant ProteinsGenes
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